This antibody solution should be stored in small working aliquots, for example, 25 ul, so they are subjected to fewer freeze-thaw cycles that can denature the antibodies. During vitrification, the solution solidifies without crystal formation and thus the structural integrity of the antibodies is maintained.įor increased stability, glycerol or ethylene glycol can be added to a final concentration of 50% and the antibody can then be stored at -20☌.

However, instead of ice crystal formation, vitrification typically occurs, as in the cryopreservation of cells/embryos with DMSO. When the storage temperature is below the freezing point for a cryoprotectant-antibody solution, the solution will solidify. (Note: ethylene glycol is toxic, and must be handled with care.) Glycerol and ethylene glycol freezing points (in Celsius) at different concentrations (in percentage %) in water. Table 1 summarizes common antibody storage conditions and other characteristics. Many commercially available antibody reagents contain one or more additives discussed below that are intended to increase the stability of the antibodies during long-term storage. Antibodies must be stored at appropriate temperature and pH ranges, and frequently, in the presence of concentrated (~1 M) substances like glycerol or sucrose, in order to retain activity and prevent aggregation. Nevertheless, some general guidelines can be applied to increase the shelf-life of your antibodies in storage.

For example, Laskowski TJ et al found that their 17-color flow cytometry panel antibody mixes remained stable for up to 15 days at 4☌ while CyTOF antibody cocktails had a maximum storage time of 3 days at 4☌. However, optimal conditions for storage are unique to each antibody. A number of diagnostic antibodies have been shown to maintain their functionalities after 12-26 years of storage at 4☌. Antibody storage 'shelf life' may range from several weeks to many years depending on both the intrinsic properties of the antibody and the storage conditions.

Cant open fxp in serum 2. 8